PubMed
Tafere Abrha G et al., Journal of bioscience and bioengineering, 2023 Oct;136(4):270-277Tafere Abrha G et al., Journal of bioscience and bioengineering, 2023 Oct;136(4):270-277
Indirect
RT-PCR - wt vs TF overexpression
Positive
BY4742
Stress
YPD medium + 35 mM furfural
N/A
Indirect
RNA-seq analysis - TF Over-expression vs WT
Positive
BY4742
Stress
Pre-culture for RNA-seq / growth assays: YPD; 30 degrees C; 200 RPM; Cells grown to OD600 in fresh 40 mL YPD before furfural addition. 35 mM furfural added to YPD at OD600. Furfural-treated cells harvested at 2 h, 8 h, and 16 h post-addition;