S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Group	Environmental Condition	Log2FC
Ume6p	YBR072W	PubMed Williams RM et al., Proceedings of the National Academy of Sciences of the United States of America, 2002 Oct 15;99(21):13431-6	Indirect	Microarray analysis - wt vs TF mutant	Negative	SK1	Carbon source quality/availability	Cells grown in YPAcetate medium to late log phase	N/A
			Indirect	Microarray analysis - wt vs TF mutant	Negative	W303	Unstressed log-phase growth (control)	Cells grown in YPD medium to late log phase	N/A
		PubMed Lardenois A et al., Molecular genetics and genomics : MGG, 2015 Oct;290(5):2031-46	Indirect	Microarray analysis - WT vs TF Deletion	Negative	SK1	Carbon source quality/availability	Grown in rich media with acetate (YPA) under standard conditions. Transcription factor gene deletion.	-2.339553
		PubMed van Heusden GPH, Genomics, 2023 Sep;115(5):110672	Indirect	RNA-seq analysis - WT vs TF Deletion	Negative	BY4741	Ion/metal/phosphate/sulfur/vitamin availability	Grown overnight at 30 °C in phosphate-free YNB medium supplemented with 7.2 mM potassium phosphate (pH 5.8), 20 mg/L histidine, leucine, methionine, and uracil, and 50 mM KCl, then used to inoculate 50 mL cultures at OD620 = 0.1 and grown at 30 °C, 180 rpm to OD620 = 0.5. Transcription factor gene deletion.	-1.12320455089806
		PubMed Harris A et al., Genetics, 2023 Oct 4;225(2):	Indirect	RNA-seq analysis - WT vs TF Deletion	Positive	SK1	Unstressed log-phase growth (control)	Mitotic, 30min: Cells were in mitotic, log-phase growth. They were grown in YPD (1% yeast extract, 2% peptone, 2% dextrose, 22.4 mg/L uracil, 80 mg/L tryptophan) for ~24 h to saturation, then back-diluted into fresh YPD to OD600 = 0.2 and grown ~3 h to log phase (OD600 ≥ 0.5). Once in log phase, β-estradiol (40 nM) was added, followed 30 min later by auxin (200 µM) at t=0min. Sampled at 30 min.	0.741346
			Indirect	RNA-seq analysis - WT vs TF Under-expression	Negative	SK1	Cell cycle/morphology	Meiotic 0.5h: Cells were transitioning from premeiotic starvation into meiosis. They were grown in YPD for ~24 h to saturation, then inoculated into BYTA (1% yeast extract, 2% bacto tryptone, 1% potassium acetate, 50 mM potassium phthalate) at OD600 = 0.25 and grown 16–18 h at 30 °C to OD600 ≥ 5. Cells were then pelleted, washed with sterile water, and resuspended in SPM (2% KOAc, 0.02% raffinose, amino acid supplements, pH 7) at OD600 = 1.85, shaken at 30 °C and 275 rpm. At 0.5 h in SPM, β-estradiol (5 nM) and auxin (200 µM) were added simultaneously, followed by CuSO4 (50 µM) at 2 h to trigger IME1/4 expression and meiotic entry. Sampled at 0.5 h.	-1.018074
			Indirect	RNA-seq analysis - WT vs TF Under-expression	Negative	SK1	Unstressed log-phase growth (control)	Mitotic, 15min: Cells were in mitotic, log-phase growth. They were grown in YPD (1% yeast extract, 2% peptone, 2% dextrose, 22.4 mg/L uracil, 80 mg/L tryptophan) for ~24 h to saturation, then back-diluted into fresh YPD to OD600 = 0.2 and grown ~3 h to log phase (OD600 ≥ 0.5). Once in log phase, β-estradiol (40 nM) was added, followed 30 min later by auxin (200 µM) at t=0min. Sampled at 15 min.	-0.4033
			Indirect	RNA-seq analysis - WT vs TF Under-expression	Negative	SK1	Cell cycle/morphology	Meiotic 2.5h: Cells were transitioning from premeiotic starvation into meiosis. They were grown in YPD for ~24 h to saturation, then inoculated into BYTA (1% yeast extract, 2% bacto tryptone, 1% potassium acetate, 50 mM potassium phthalate) at OD600 = 0.25 and grown 16–18 h at 30 °C to OD600 ≥ 5. Cells were then pelleted, washed with sterile water, and resuspended in SPM (2% KOAc, 0.02% raffinose, amino acid supplements, pH 7) at OD600 = 1.85, shaken at 30 °C and 275 rpm. At 0.5 h in SPM, β-estradiol (5 nM) and auxin (200 µM) were added simultaneously, followed by CuSO4 (50 µM) at 2 h to trigger IME1/4 expression and meiotic entry. Sampled at 2.5 h.	-1.864312