PubMed
Shu WJ et al., Nucleic acids research, 2020 Sep 4;48(15):8360-8373Shu WJ et al., Nucleic acids research, 2020 Sep 4;48(15):8360-8373
Indirect
RNA-seq analysis - WT vs TF Deletion
Negative
BY4741
Steady state carbon-limited chemostat cultures operated at a dilution rate of 0.05 per h by continuous addition of medium (feed concentration of glucose, 10 g/liter) at 30°C. pH controlled at 5, aerated with 0.5 volume of air/volume of liquid/min and with 800 rpm agitation. Transcription factor gene deletion.
-0.576091654934781
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
BY4741
Ethanol medium - Overexpression media lacked leucine to ensure plasmid selection. The cultures grown for 70 h in ethanol medium were diluted twice with a fresh medium to ensure continuous growth.
0.361799036195995
Indirect
RNA-seq analysis - WT vs TF Deletion
Negative
BY4741
Grown in YPD to OD600 of 0.8 at 30 ºC. Transcription factor gene deletion.
-0.576091654934781
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
BY4741
Grown in YPD to OD600 of 0.8 at 30 ºC. Treated with rapamycin for 2h (100 nM). Transcription factor gene deletion.