PubMed
Galeote VA et al., Current genetics, 2007 Aug;52(2):55-63Galeote VA et al., Current genetics, 2007 Aug;52(2):55-63
Indirect
Microarray analysis - WT vs TF Deletion
Negative
BY4741
Yeast strains were maintained and grown on yeast–peptone–dextrose (YPD) medium (1% Bacto yeast extract, 2% Bacto peptone, 2% glucose). Transcriptome after application of an ethanol shock (7% v/v) for 30 min.
-1.137503524
PubMed
Cromie GA et al., G3 (Bethesda, Md.), 2017 Aug 7;7(8):2845-2854Cromie GA et al., G3 (Bethesda, Md.), 2017 Aug 7;7(8):2845-2854
Indirect
RNA-seq analysis - TF Over-expression vs WT
Positive
F45
Yeast cultures were grown in synthetic (SM) or rich (YP) medium to OD 600 of 1.0.
0.178376898682032
Indirect
RNA-seq analysis - TF Over-expression vs WT
Positive
F45
4 days of growth on YPD (with 2% glucose plus G418 to maintain plasmid selection) plates at 30 °C. Transcription factor gene overexpression.
0.178376898682032
PubMed
Cromie GA et al., PloS one, 2024;19(12):e0311061Cromie GA et al., PloS one, 2024;19(12):e0311061
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
F13
Grown on YPD (2% glucose) agar plates at 30° C for 5 days, 3–5 colonies were pooled per sample. The structured outside region of the colonies was used for the wild-type. Transcription factor gene deletion.