S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Group	Environmental Condition	Log2FC
Rim101p	YDR040C	PubMed Zhu H et al., Rapid communications in mass spectrometry : RCM, 2002;16(22):2115-23	Indirect	lacZ - wt vs TF mutant	Positive	S288c	Stress	Alkaline pH stress; Cells cultivated in SC medium at pH 8	N/A
		PubMed Lamb TM et al., Molecular and cellular biology, 2003 Jan;23(2):677-86	Indirect	lacZ - wt vs TF mutant	Positive	SK1	Stress	Acidic pH; cells expressing Ena1-lacZ grown to exponential phase in YPD medium at pH 4	N/A
			Indirect	lacZ - wt vs TF mutant	Positive	SK1	Stress	Alkaline pH; cells expressing Ena1-lacZ grown to exponential phase in YPD medium at pH 8	N/A
		PubMed Platara M et al., The Journal of biological chemistry, 2006 Dec 1;281(48):36632-42	Indirect	lacZ - wt vs TF mutant	Positive	DBY746	Stress	Cells grown in YPD until A660 of 0.5 to 0.7, alkaline stress was provoked by resuspending cells in YPD containing 50 nM TAPS adjusted to pH 8.0 by adding KOH	N/A
			Indirect	lacZ - wt vs TF mutant	Positive	DBY746	Unstressed log-phase growth (control)	Cells grown in YPD until A660 of 0.5 to 0.7, resuspended in YPD medium adjusted to pH 6.3	N/A
		PubMed Yazawa H et al., Yeast (Chichester, England), 2009 Mar;26(3):167-84	Indirect	RT-PCR - wt vs TF mutant	Positive	YHU3201	Stress	Alkaline stress - 240 mM K2HPO4	N/A
		PubMed Marqués MC et al., FEMS yeast research, 2015 Jun;15(4):fov017	Indirect	Western blot - wt vs TF mutant	Positive	S288c	Stress	Strains treated with 0.5 M NaCl for 20 min harbouring a genomic integration of the GFP coding sequence at the ENA1 C-terminus.	N/A
		PubMed Read T et al., PLoS genetics, 2016 Jan;12(1):e1005746	Indirect	RNA-seq analysis - WT vs TF Deletion	Positive	L6441	Ion/metal/phosphate/sulfur/vitamin availability	Cells were grown in 100 ml of CSD (Chelex-treated synthetic defined medium) containing <100 nM of zinc. Cells were inoculated from overnight cultures into CSD at an initial OD600 = 0.01 and grown to mid-log phase (OD600 ≈ 0.5) before RNA isolation.	2.91600721456567
			Indirect	RNA-seq analysis - WT vs TF Deletion	Positive	L6441	Unstressed log-phase growth (control)	Grown to mid-log phase in standard YPD media. Transcription factor gene deletion.	2.91600721456567
		PubMed Anton C et al., Molecular biology of the cell, 2017 Dec 1;28(25):3672-3685	Indirect	Western blot - wt vs TF mutant	Positive	CRM67 (W303, mat a, (leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15))	Unstressed log-phase growth (control)	Levels of Ena1-GFP from its endogenous locus after Indirect of the constitutively processed Rim101C [Lamb et al.,2001] from the pRS315 plasmid. WT cells were grown O/N in selective SD (2% glucose, 0.7% Difco yeast nitrogen base without amino acids supplemented with the appropriate amino acids) media and refreshed in YPD media for 2 h	N/A
			Indirect	Western blot - wt vs TF mutant	Positive	CRM2268 (W303, mat a, chs5DELTA	Unstressed log-phase growth (control)	Levels of Ena1-GFP from its endogenous locus after Indirect of the constitutively processed Rim101C [Lamb et al.,2001] from the pRS315 plasmid. DELTAchs5 cells were grown O/N in selective SD (2% glucose, 0.7% Difco yeast nitrogen base without amino acids supplemented with the appropriate amino acids) media and refreshed in YPD media for 2 h	N/A