PubMed
Lamb TM et al., Molecular and cellular biology, 2003 Jan;23(2):677-86Lamb TM et al., Molecular and cellular biology, 2003 Jan;23(2):677-86
Indirect
Microarray analysis - WT vs TF Deletion
Positive
TLY941
Yeast growth media (yeast-peptone-dextrose [YPD], yeast-peptone-acetate, and synthetic complete) were of standard composition
N/A
PubMed
Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104
Direct
Chip-on-chip
N/A
S288c
N/A
N/A
PubMed
Shock TR et al., Eukaryotic cell, 2009 Apr;8(4):606-16Shock TR et al., Eukaryotic cell, 2009 Apr;8(4):606-16
Indirect
Microarray analysis - wt vs TF mutant
Positive
S288c
Exponential phase cells cultivated in YNB (yeast nitrogen base), 2% glucose and 2.65 g/L NH4(SO4)2
N/A
PubMed
Reimand J et al., Nucleic acids research, 2010 Aug;38(14):4768-77Reimand J et al., Nucleic acids research, 2010 Aug;38(14):4768-77
Indirect
Microarray analysis - WT vs TF Deletion
Positive
BY4741; S288C; R1158
Standard YPD conditions Dataset ewxtracted from The Longhorn Array Database (LAD). Transcription factor gene deletion.
0.32762582899566
PubMed
Read T et al., PLoS genetics, 2016 Jan;12(1):e1005746Read T et al., PLoS genetics, 2016 Jan;12(1):e1005746
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
L6441
Cells were grown in 100 ml of CSD (Chelex-treated synthetic defined medium) containing <100 nM of zinc. Cells were inoculated from overnight cultures into CSD at an initial OD600 = 0.01 and grown to mid-log phase (OD600 ≈ 0.5) before RNA isolation.
5.43316644757695
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
L6441
Grown to mid-log phase in standard YPD media. Transcription factor gene deletion.
