PubMed
Turi TG et al., The Journal of biological chemistry, 1992 Jan 25;267(3):2046-56Turi TG et al., The Journal of biological chemistry, 1992 Jan 25;267(3):2046-56
Direct
lacZ - wt vs mutant promoter sequence
N/A
S288c
N/A
N/A
PubMed
Kwast KE et al., Current genetics, 1997 Dec;32(6):377-83Kwast KE et al., Current genetics, 1997 Dec;32(6):377-83
Indirect
Northern blot - wt vs TF mutant
Negative
S288c
Semi Synthetic galactose (10g/L) medium with 0.1% Tween 80 (v/v), 20 mg of ergosterol, 350 ppm of silicone antifoam (per liter) - SSG-TEA
N/A
PubMed
Chua G et al., Proceedings of the National Academy of Sciences of the United States of America, 2006 Aug 8;103(32):12045-50Chua G et al., Proceedings of the National Academy of Sciences of the United States of America, 2006 Aug 8;103(32):12045-50
Indirect
Microarray analysis - wt vs TF overexpression
Positive
N/A
Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h
N/A
PubMed
van der Horst SC et al., Nature communications, 2025 Feb 6;16(1):1416van der Horst SC et al., Nature communications, 2025 Feb 6;16(1):1416
Indirect
RNA-seq analysis - WT vs TF Deletion
Negative
W303
60 min: RNA-seq performed in YPAD medium. Overnight cultures were diluted in fresh YPAD and grown for 3 h. Cells were then synchronized in G1 by α-factor treatment for 2 h, washed once in YPAD, and released into YPAD containing 200 mM hydroxyurea (HU). Samples were collected at t=60min: 60 min after release into HU (replication stress condition)
