PubMed
Cipollina C et al., Microbiology (Reading, England), 2008 Jan;154(Pt 1):337-346Cipollina C et al., Microbiology (Reading, England), 2008 Jan;154(Pt 1):337-346
Indirect
Microarray analysis - WT vs TF Deletion
Negative
SLY34
Unstressed log-phase growth (control)
Grown in rich medium (YPD) at 30 °C to log phase. Transcription factor gene deletion.
-0.220935
PubMed
Fang W et al., Cell reports, 2022 Apr 12;39(2):110672Fang W et al., Cell reports, 2022 Apr 12;39(2):110672
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
CEN.PK
Carbon source quality/availability
Yeast cells grown to log phase in YPL medium from 0.1 OD with switch to SL minimal medium for 3h. Transcription factor gene deletion.
0.98813569102227
PubMed
Glazier VE et al., mBio, 2023 Oct 31;14(5):e0152123Glazier VE et al., mBio, 2023 Oct 31;14(5):e0152123
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
BY4741
Unstressed log-phase growth (control)
Cultured in minimal medium (1.7 g/L YNB, 5 g/L ammonium sulfate, 20 g/L glucose, 80 mg/L histidine, 80 mg/L methionine, 80 mg/L uracil and 240 mg/L leucine) with 200 rpm orbital shaking at 30°C, pH 5.5, until the mid-exponential phase (OD600 = 0.6). Transcription factor gene deletion.
