PubMed
Lee TI et al., Science (New York, N.Y.), 2002 Oct 25;298(5594):799-804Lee TI et al., Science (New York, N.Y.), 2002 Oct 25;298(5594):799-804
Direct
Chip-on-chip
N/A
S288c
Unstressed log-phase growth (control)
YPD medium; mid-log phase
N/A
PubMed
Shu WJ et al., Nucleic acids research, 2020 Sep 4;48(15):8360-8373Shu WJ et al., Nucleic acids research, 2020 Sep 4;48(15):8360-8373
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
BY4741
Carbon source quality/availability
Ethanol medium - Overexpression media lacked leucine to ensure plasmid selection. The cultures grown for 70 h in ethanol medium were diluted twice with a fresh medium to ensure continuous growth.
0.5323957470428
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
BY4741
Nitrogen source quality/availability
Grown in YPD to OD600 of 0.8 at 30 ÂșC. Treated with rapamycin for 2h (100 nM). Transcription factor gene deletion.