PubMed
Cipollina C et al., Microbiology (Reading, England), 2008 Jan;154(Pt 1):337-346Cipollina C et al., Microbiology (Reading, England), 2008 Jan;154(Pt 1):337-346
Indirect
Microarray analysis - WT vs TF Deletion
Positive
SLY34
Unstressed log-phase growth (control)
Grown in rich medium (YPD) at 30 °C to log phase. Transcription factor gene deletion.
0.063139
PubMed
Shu WJ et al., Nucleic acids research, 2020 Sep 4;48(15):8360-8373Shu WJ et al., Nucleic acids research, 2020 Sep 4;48(15):8360-8373
Indirect
RNA-seq analysis - WT vs TF Deletion
Negative
BY4741
Carbon source quality/availability
Ethanol medium - Overexpression media lacked leucine to ensure plasmid selection. The cultures grown for 70 h in ethanol medium were diluted twice with a fresh medium to ensure continuous growth.
-0.292219221606632
Indirect
RNA-seq analysis - WT vs TF Deletion
Negative
BY4741
Nitrogen source quality/availability
Grown in YPD to OD600 of 0.8 at 30 ºC. Treated with rapamycin for 2h (100 nM). Transcription factor gene deletion.
-0.292219221606632
PubMed
Couvillion M et al., eLife, 2022 May 16;11():Couvillion M et al., eLife, 2022 May 16;11():
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
BY4741
Unstressed log-phase growth (control)
Overnight cultures grown in YPD medium were diluted to OD600 = 0.05 in 1 L YPD and incubated at 30 °C with 200 rpm shaking to mid-log phase (OD600 = 0.6–0.8). Transcription factor gene deletion.
