PubMed
Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314
Direct
ChIP-exo
N/A
BY4741
YPD medium, 25.C
N/A
PubMed
Harris A et al., Genetics, 2023 Oct 4;225(2):Harris A et al., Genetics, 2023 Oct 4;225(2):
Indirect
RNA-seq analysis - WT vs TF Deletion
Negative
SK1
Mitotic, 30min: Cells were in mitotic, log-phase growth. They were grown in YPD (1% yeast extract, 2% peptone, 2% dextrose, 22.4 mg/L uracil, 80 mg/L tryptophan) for ~24 h to saturation, then back-diluted into fresh YPD to OD600 = 0.2 and grown ~3 h to log phase (OD600 ≥ 0.5). Once in log phase, β-estradiol (40 nM) was added, followed 30 min later by auxin (200 µM) at t=0min. Sampled at 30 min.
