PubMed
Cipollina C et al., Microbiology (Reading, England), 2008 Jan;154(Pt 1):337-346Cipollina C et al., Microbiology (Reading, England), 2008 Jan;154(Pt 1):337-346
Indirect
Microarray analysis - WT vs TF Deletion
Positive
SLY34
Grown in rich medium (YPD) at 30 °C to log phase. Transcription factor gene deletion.
0.168265
PubMed
Couvillion M et al., eLife, 2022 May 16;11():Couvillion M et al., eLife, 2022 May 16;11():
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
BY4741
Overnight cultures grown in YPD medium were diluted to OD600 = 0.05 in 1 L YPD and incubated at 30 °C with 200 rpm shaking to mid-log phase (OD600 = 0.6–0.8). Transcription factor gene deletion.
1.60309610921027
PubMed
Glazier VE et al., mBio, 2023 Oct 31;14(5):e0152123Glazier VE et al., mBio, 2023 Oct 31;14(5):e0152123
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
BY4741
Cultured in minimal medium (1.7 g/L YNB, 5 g/L ammonium sulfate, 20 g/L glucose, 80 mg/L histidine, 80 mg/L methionine, 80 mg/L uracil and 240 mg/L leucine) with 200 rpm orbital shaking at 30°C, pH 5.5, until the mid-exponential phase (OD600 = 0.6). Transcription factor gene deletion.