The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.
Transcription
Factor |
Target
ORF/Genes |
References |
Evidence
Code |
Evidence
Experiment |
Association
Type |
Strain |
Environmental
Group |
Environmental
Condition |
Log2FC |
|
Ume6p
|
YGR109C
|
PubMed 
Lardenois A et al., Molecular genetics and genomics : MGG, 2015 Oct;290(5):2031-46Lardenois A et al., Molecular genetics and genomics : MGG, 2015 Oct;290(5):2031-46 |
Indirect |
Microarray analysis - wt vs TF mutant |
Positive |
S288c |
Cell cycle/morphology |
SPII 8h growth |
N/A |
|
PubMed
Harris A et al., Genetics, 2023 Oct 4;225(2):Harris A et al., Genetics, 2023 Oct 4;225(2): |
Indirect |
RNA-seq analysis - WT vs TF Under-expression |
Negative |
SK1 |
Cell cycle/morphology |
Meiotic 0.5h: Cells were transitioning from premeiotic starvation into meiosis. They were grown in YPD for ~24 h to saturation, then inoculated into BYTA (1% yeast extract, 2% bacto tryptone, 1% potassium acetate, 50 mM potassium phthalate) at OD600 = 0.25 and grown 16–18 h at 30 °C to OD600 ≥ 5. Cells were then pelleted, washed with sterile water, and resuspended in SPM (2% KOAc, 0.02% raffinose, amino acid supplements, pH 7) at OD600 = 1.85, shaken at 30 °C and 275 rpm. At 0.5 h in SPM, β-estradiol (5 nM) and auxin (200 µM) were added simultaneously, followed by CuSO4 (50 µM) at 2 h to trigger IME1/4 expression and meiotic entry. Sampled at 0.5 h. |
-3.079867 |
| Indirect |
RNA-seq analysis - WT vs TF Under-expression |
Negative |
SK1 |
Cell cycle/morphology |
Meiotic 2.5h: Cells were transitioning from premeiotic starvation into meiosis. They were grown in YPD for ~24 h to saturation, then inoculated into BYTA (1% yeast extract, 2% bacto tryptone, 1% potassium acetate, 50 mM potassium phthalate) at OD600 = 0.25 and grown 16–18 h at 30 °C to OD600 ≥ 5. Cells were then pelleted, washed with sterile water, and resuspended in SPM (2% KOAc, 0.02% raffinose, amino acid supplements, pH 7) at OD600 = 1.85, shaken at 30 °C and 275 rpm. At 0.5 h in SPM, β-estradiol (5 nM) and auxin (200 µM) were added simultaneously, followed by CuSO4 (50 µM) at 2 h to trigger IME1/4 expression and meiotic entry. Sampled at 2.5 h. |
-3.318832 |
| Indirect |
RNA-seq analysis - WT vs TF Under-expression |
Positive |
SK1 |
Cell cycle/morphology |
Meiotic 3h: Cells were transitioning from premeiotic starvation into meiosis. They were grown in YPD for ~24 h to saturation, then inoculated into BYTA (1% yeast extract, 2% bacto tryptone, 1% potassium acetate, 50 mM potassium phthalate) at OD600 = 0.25 and grown 16–18 h at 30 °C to OD600 ≥ 5. Cells were then pelleted, washed with sterile water, and resuspended in SPM (2% KOAc, 0.02% raffinose, amino acid supplements, pH 7) at OD600 = 1.85, shaken at 30 °C and 275 rpm. At 0.5 h in SPM, β-estradiol (5 nM) and auxin (200 µM) were added simultaneously, followed by CuSO4 (50 µM) at 2 h to trigger IME1/4 expression and meiotic entry. Sampled at 3 h. |
3.796811 |
|
