PubMed
Chua G et al., Proceedings of the National Academy of Sciences of the United States of America, 2006 Aug 8;103(32):12045-50Chua G et al., Proceedings of the National Academy of Sciences of the United States of America, 2006 Aug 8;103(32):12045-50
Indirect
Microarray analysis - wt vs TF overexpression
Positive
N/A
Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h
N/A
PubMed
Lotito L et al., European journal of pharmacology, 2009 Jan 28;603(1-3):29-36Lotito L et al., European journal of pharmacology, 2009 Jan 28;603(1-3):29-36
Indirect
RT-PCR - wt vs TF mutant
Positive
S288c
Control - 0.05% dimethyl sulfoxide
N/A
PubMed
Davidson MB et al., The EMBO journal, 2012 Feb 15;31(4):895-907Davidson MB et al., The EMBO journal, 2012 Feb 15;31(4):895-907
Indirect
Western blot - wt vs TF mutant
Positive
S288c
Logarithmically growing cells arrested in G1 with alpha factor