PubMed
Reimand J et al., Nucleic acids research, 2010 Aug;38(14):4768-77Reimand J et al., Nucleic acids research, 2010 Aug;38(14):4768-77
Indirect
Microarray analysis - WT vs TF Deletion
Positive
BY4741; S288C; R1158
Unstressed log-phase growth (control)
Standard YPD conditions Dataset ewxtracted from The Longhorn Array Database (LAD). Transcription factor gene deletion.
0.97736356075638
PubMed
Harris A et al., Genetics, 2023 Oct 4;225(2):Harris A et al., Genetics, 2023 Oct 4;225(2):
Indirect
RNA-seq analysis - WT vs TF Under-expression
Positive
SK1
Cell cycle/morphology
Meiotic 2h: Cells were transitioning from premeiotic starvation into meiosis. They were grown in YPD for ~24 h to saturation, then inoculated into BYTA (1% yeast extract, 2% bacto tryptone, 1% potassium acetate, 50 mM potassium phthalate) at OD600 = 0.25 and grown 16–18 h at 30 °C to OD600 ≥ 5. Cells were then pelleted, washed with sterile water, and resuspended in SPM (2% KOAc, 0.02% raffinose, amino acid supplements, pH 7) at OD600 = 1.85, shaken at 30 °C and 275 rpm. At 0.5 h in SPM, β-estradiol (5 nM) and auxin (200 µM) were added simultaneously, followed by CuSO4 (50 µM) at 2 h to trigger IME1/4 expression and meiotic entry. Sampled at 2 h.
