S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition	Log2FC
Ime1p	YJL106W	PubMed Mitchell AP et al., Molecular and cellular biology, 1990 May;10(5):2104-10	Indirect	Northern blot - wt vs TF mutant	Positive	S288c	SPO (sporulation) medium	N/A
		PubMed Smith HE et al., Molecular and cellular biology, 1990 Dec;10(12):6103-13	Indirect	Northern blot - wt vs TF mutant	Positive	S288c	YEPAc medium (2% acetate)	N/A
		PubMed Smith HE et al., Molecular and cellular biology, 1989 May;9(5):2142-52	Indirect	Northern blot - wt vs TF mutant	Negative	S288c	Sporulation medium	N/A
			Indirect	Northern blot - wt vs TF mutant	Negative	S288c	YEPac medium	N/A
		PubMed Bowdish KS et al., Molecular and cellular biology, 1993 Apr;13(4):2172-81	Direct	lacZ - wt vs mutant promoter sequence	Positive	S288c	growth in sporulation medium for 8h	N/A
		PubMed Pnueli L et al., Molecular and cellular biology, 2004 Jun;24(12):5197-208	Direct	ChIP	N/A	S288c	Yeast cells were grown in medium with acetate (SA)	N/A
		PubMed Gurevich V et al., PloS one, 2010 Jun 8;5(6):e11005	Direct	RT-PCR - wt vs mutant promoter sequence	Positive	S288c	SPO (sporulation) medium	N/A
		PubMed Strudwick N et al., Molecular and cellular biology, 2010 Dec;30(23):5514-30	Indirect	Northern blot - wt vs TF mutant	Positive	S288c	SPO (sporulation) medium	N/A
		PubMed Piccirillo S et al., Genetics, 2015 Dec;201(4):1427-38	Indirect	lacZ - wt vs TF mutant	Positive	S288c	6-day spots on YNA-2 plates	N/A
		PubMed Park ZM et al., Genetics, 2023 May 4;224(1):	Indirect	RNA-seq analysis - TF Over-expression vs WT	Positive	S288c	12 hours: RNA-seq was performed under sporulation (meiotic induction) conditions. Overnight YPD cultures were diluted to OD600 of 0.1 in YPA (1% yeast extract, 2% peptone, 1% potassium acetate) and grown 16-18 h at 30°C. Cells were then resuspended at OD600 of 0.5 in 1% potassium acetate (Spo medium) supplemented with uracil, histidine, and leucine, and incubated at 26°C. RNA-seq samples were collected at 12 hours post-transfer to Spo medium.	3.455045
			Indirect	RNA-seq analysis - TF Over-expression vs WT	Positive	S288c	2 hours: RNA-seq was performed under sporulation (meiotic induction) conditions. Overnight YPD cultures were diluted to OD600 of 0.1 in YPA (1% yeast extract, 2% peptone, 1% potassium acetate) and grown 16-18 h at 30°C. Cells were then resuspended at OD600 of 0.5 in 1% potassium acetate (Spo medium) supplemented with uracil, histidine, and leucine, and incubated at 26°C. RNA-seq samples were collected at 2 hours post-transfer to Spo medium.	2.402191
			Indirect	RNA-seq analysis - TF Over-expression vs WT	Positive	S288c	4 hours: RNA-seq was performed under sporulation (meiotic induction) conditions. Overnight YPD cultures were diluted to OD600 of 0.1 in YPA (1% yeast extract, 2% peptone, 1% potassium acetate) and grown 16-18 h at 30°C. Cells were then resuspended at OD600 of 0.5 in 1% potassium acetate (Spo medium) supplemented with uracil, histidine, and leucine, and incubated at 26°C. RNA-seq samples were collected at 4 hours post-transfer to Spo medium.	2.68405
			Indirect	RNA-seq analysis - TF Over-expression vs WT	Positive	S288c	6 hours: RNA-seq was performed under sporulation (meiotic induction) conditions. Overnight YPD cultures were diluted to OD600 of 0.1 in YPA (1% yeast extract, 2% peptone, 1% potassium acetate) and grown 16-18 h at 30°C. Cells were then resuspended at OD600 of 0.5 in 1% potassium acetate (Spo medium) supplemented with uracil, histidine, and leucine, and incubated at 26°C. RNA-seq samples were collected at 6 hours post-transfer to Spo medium.	3.092711
			Indirect	RNA-seq analysis - TF Over-expression vs WT	Positive	S288c	8 hours: RNA-seq was performed under sporulation (meiotic induction) conditions. Overnight YPD cultures were diluted to OD600 of 0.1 in YPA (1% yeast extract, 2% peptone, 1% potassium acetate) and grown 16-18 h at 30°C. Cells were then resuspended at OD600 of 0.5 in 1% potassium acetate (Spo medium) supplemented with uracil, histidine, and leucine, and incubated at 26°C. RNA-seq samples were collected at 8 hours post-transfer to Spo medium.	2.641275
		PubMed Park ZM et al., PLoS genetics, 2023 Aug;19(8):e1010898	Indirect	Microarray hibridization - wt vs TF overexpression	Positive	S288c	YPAc sporulation medium	N/A