PubMed
Shu WJ et al., Nucleic acids research, 2020 Sep 4;48(15):8360-8373Shu WJ et al., Nucleic acids research, 2020 Sep 4;48(15):8360-8373
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
BY4741
Carbon source quality/availability
Ethanol medium - Overexpression media lacked leucine to ensure plasmid selection. The cultures grown for 70 h in ethanol medium were diluted twice with a fresh medium to ensure continuous growth.
0.558575191451306
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
BY4741
Nitrogen source quality/availability
Grown in YPD to OD600 of 0.8 at 30 ºC. Treated with rapamycin for 2h (100 nM). Transcription factor gene deletion.
0.558575191451306
PubMed
Glazier VE et al., mBio, 2023 Oct 31;14(5):e0152123Glazier VE et al., mBio, 2023 Oct 31;14(5):e0152123
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
BY4741
Unstressed log-phase growth (control)
Cultured in minimal medium (1.7 g/L YNB, 5 g/L ammonium sulfate, 20 g/L glucose, 80 mg/L histidine, 80 mg/L methionine, 80 mg/L uracil and 240 mg/L leucine) with 200 rpm orbital shaking at 30°C, pH 5.5, until the mid-exponential phase (OD600 = 0.6). Transcription factor gene deletion.
