PubMed
Shu WJ et al., Nucleic acids research, 2020 Sep 4;48(15):8360-8373Shu WJ et al., Nucleic acids research, 2020 Sep 4;48(15):8360-8373
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
BY4741
Steady state carbon-limited chemostat cultures operated at a dilution rate of 0.05 per h by continuous addition of medium (feed concentration of glucose, 10 g/liter) at 30°C. pH controlled at 5, aerated with 0.5 volume of air/volume of liquid/min and with 800 rpm agitation. Transcription factor gene deletion.
0.755310736347303
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
BY4741
Grown in YPD to OD600 of 0.8 at 30 ºC. Transcription factor gene deletion.
0.755310736347303
PubMed
Fang W et al., Cell reports, 2022 Apr 12;39(2):110672Fang W et al., Cell reports, 2022 Apr 12;39(2):110672
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
CEN.PK
Yeast cells grown to log phase in YPL medium from 0.1 OD with switch to SL minimal medium for 3h. Transcription factor gene deletion.
2.01063786258359
PubMed
Couvillion M et al., eLife, 2022 May 16;11():Couvillion M et al., eLife, 2022 May 16;11():
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
BY4741
Overnight cultures grown in YPD medium were diluted to OD600 = 0.05 in 1 L YPD and incubated at 30 °C with 200 rpm shaking to mid-log phase (OD600 = 0.6–0.8). Transcription factor gene deletion.