PubMed
Capaldi AP et al., Nature genetics, 2008 Nov;40(11):1300-6Capaldi AP et al., Nature genetics, 2008 Nov;40(11):1300-6
Indirect
Microarray analysis - wt vs TF mutant
Positive
S288c
Stress
YPD medium + 0,4M KCl (salt stress)
N/A
PubMed
Nussbaum I et al., PloS one, 2014;9(10):e111505Nussbaum I et al., PloS one, 2014;9(10):e111505
Indirect
Luciferase - wt vs TF mutant
Positive
S288c
Stress
Cells were grown for 12h in SD medium supplemented with 1mM FeCl2. The luciferase activities were determined and normalized to protein concentration. Cells harboring the empty vector served as a control. Luciferase activities were determined in DELTAyap5 cells harboring plasmids pCCC1-LUX (CCC1 promoter) or pYRE-LUX (hybrid promoter that consists of the two YREs of the CCC1 promoter and the LEU1 cor