PubMed
Reimand J et al., Nucleic acids research, 2010 Aug;38(14):4768-77Reimand J et al., Nucleic acids research, 2010 Aug;38(14):4768-77
Indirect
Microarray analysis - WT vs TF Deletion
Positive
BY4741; S288C; R1158
Unstressed log-phase growth (control)
Standard YPD conditions Dataset ewxtracted from The Longhorn Array Database (LAD). Transcription factor gene deletion.
0.61077836779404
PubMed
He BZ et al., eLife, 2017 May 9;6():He BZ et al., eLife, 2017 May 9;6():
Indirect
RNA-seq analysis - WT vs TF Deletion
Negative
K699
Unstressed log-phase growth (control)
100 ml of cell culture grown to OD600nm (600 nm) of 1.5.
-0.367255779054003
Indirect
RNA-seq analysis - WT vs TF Deletion
Negative
K699
Ion/metal/phosphate/sulfur/vitamin availability
Grown in phosphate-free SC medium (Phosphate starvation) at 30°C with shaking. All transcriptional profiling experiments were done in the pho80∆ background. Transcription factor gene deletion.
Grown overnight at 30 °C in phosphate-free YNB medium supplemented with 7.2 mM potassium phosphate (pH 5.8), 20 mg/L histidine, leucine, methionine, and uracil, and 50 mM KCl, then used to inoculate 50 mL cultures at OD620 = 0.1 and grown at 30 °C, 180 rpm to OD620 = 0.5. Transcription factor gene deletion.
