PubMed
Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104
Direct
Chip-on-chip
N/A
S288c
SC medium; mid-log phase
N/A
PubMed
Shu WJ et al., Nucleic acids research, 2020 Sep 4;48(15):8360-8373Shu WJ et al., Nucleic acids research, 2020 Sep 4;48(15):8360-8373
Indirect
RNA-seq analysis - WT vs TF Deletion
Negative
BY4741
Ethanol medium - Overexpression media lacked leucine to ensure plasmid selection. The cultures grown for 70 h in ethanol medium were diluted twice with a fresh medium to ensure continuous growth.
-1.22518317798392
Indirect
RNA-seq analysis - WT vs TF Deletion
Negative
BY4741
Grown in YPD to OD600 of 0.8 at 30 ÂșC. Treated with rapamycin for 2h (100 nM). Transcription factor gene deletion.