S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition	Log2FC
Ume6p	YML042W	PubMed Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104	Direct	Chip-on-chip	N/A	S288c	YPD medium; mid-log phase	N/A
		PubMed Yukawa M et al., Bioscience, biotechnology, and biochemistry, 2009 Feb;73(2):378-84	Indirect	Northern blot - wt vs TF mutant	Negative	MYC500	YPA 2% potassium acetate instead of the glucose in YPD) 28.C	N/A
		PubMed Reimand J et al., Nucleic acids research, 2010 Aug;38(14):4768-77	Indirect	Microarray analysis - WT vs TF Deletion	Negative	BY4741; S288C; R1158	Standard YPD conditions Dataset ewxtracted from The Longhorn Array Database (LAD). Transcription factor gene deletion.	-0.39186615700969
		PubMed Lardenois A et al., Molecular genetics and genomics : MGG, 2015 Oct;290(5):2031-46	Indirect	Microarray analysis - WT vs TF Deletion	Negative	SK1	Grown in sporulation medium (SPII for 10 h) under standard conditions. Transcription factor gene deletion.	-0.578428
			Indirect	Microarray analysis - WT vs TF Deletion	Positive	SK1	Grown in sporulation medium (SPII for 4 h) under standard conditions. Transcription factor gene deletion.	0.179873
			Indirect	Microarray analysis - WT vs TF Deletion	Negative	SK1	Grown in sporulation medium (SPII for 8 h) under standard conditions. Transcription factor gene deletion.	-0.537342
			Indirect	Microarray analysis - WT vs TF Deletion	Negative	SK1	Grown in rich media with acetate (YPA) under standard conditions. Transcription factor gene deletion.	-0.429609
			Indirect	Microarray analysis - WT vs TF Deletion	Negative	SK1	Grown in rich media with glucose (YPD) under standard conditions. Transcription factor gene deletion.	-0.913885
		PubMed van Heusden GPH, Genomics, 2023 Sep;115(5):110672	Indirect	RNA-seq analysis - WT vs TF Deletion	Negative	BY4741	Grown overnight at 30 °C in phosphate-free YNB medium supplemented with 7.2 mM potassium phosphate (pH 5.8), 20 mg/L histidine, leucine, methionine, and uracil, and 50 mM KCl, then used to inoculate 50 mL cultures at OD620 = 0.1 and grown at 30 °C, 180 rpm to OD620 = 0.5. Transcription factor gene deletion.	-2.2851009028068
		PubMed Harris A et al., Genetics, 2023 Oct 4;225(2):	Indirect	RNA-seq analysis - WT vs TF Deletion	Negative	SK1	Mitotic, 30min: Cells were in mitotic, log-phase growth. They were grown in YPD (1% yeast extract, 2% peptone, 2% dextrose, 22.4 mg/L uracil, 80 mg/L tryptophan) for ~24 h to saturation, then back-diluted into fresh YPD to OD600 = 0.2 and grown ~3 h to log phase (OD600 ≥ 0.5). Once in log phase, β-estradiol (40 nM) was added, followed 30 min later by auxin (200 µM) at t=0min. Sampled at 30 min.	-1.942232
			Indirect	RNA-seq analysis - WT vs TF Under-expression	Negative	SK1	Mitotic, 120min: Cells were in mitotic, log-phase growth. They were grown in YPD (1% yeast extract, 2% peptone, 2% dextrose, 22.4 mg/L uracil, 80 mg/L tryptophan) for ~24 h to saturation, then back-diluted into fresh YPD to OD600 = 0.2 and grown ~3 h to log phase (OD600 ≥ 0.5). Once in log phase, β-estradiol (40 nM) was added, followed 30 min later by auxin (200 µM) at t=0min. Sampled at 120 min.	-1.236775
			Indirect	RNA-seq analysis - WT vs TF Under-expression	Negative	SK1	Mitotic, 15min: Cells were in mitotic, log-phase growth. They were grown in YPD (1% yeast extract, 2% peptone, 2% dextrose, 22.4 mg/L uracil, 80 mg/L tryptophan) for ~24 h to saturation, then back-diluted into fresh YPD to OD600 = 0.2 and grown ~3 h to log phase (OD600 ≥ 0.5). Once in log phase, β-estradiol (40 nM) was added, followed 30 min later by auxin (200 µM) at t=0min. Sampled at 15 min.	-1.350315
			Indirect	RNA-seq analysis - WT vs TF Under-expression	Negative	SK1	Mitotic, 30min: Cells were in mitotic, log-phase growth. They were grown in YPD (1% yeast extract, 2% peptone, 2% dextrose, 22.4 mg/L uracil, 80 mg/L tryptophan) for ~24 h to saturation, then back-diluted into fresh YPD to OD600 = 0.2 and grown ~3 h to log phase (OD600 ≥ 0.5). Once in log phase, β-estradiol (40 nM) was added, followed 30 min later by auxin (200 µM) at t=0min. Sampled at 30 min.	-0.929933
			Indirect	RNA-seq analysis - WT vs TF Under-expression	Negative	SK1	Mitotic, 60min: Cells were in mitotic, log-phase growth. They were grown in YPD (1% yeast extract, 2% peptone, 2% dextrose, 22.4 mg/L uracil, 80 mg/L tryptophan) for ~24 h to saturation, then back-diluted into fresh YPD to OD600 = 0.2 and grown ~3 h to log phase (OD600 ≥ 0.5). Once in log phase, β-estradiol (40 nM) was added, followed 30 min later by auxin (200 µM) at t=0min. Sampled at 60 min.	-0.593937