S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition	Log2FC
Pho4p	YML123C	PubMed Ogawa N et al., Molecular biology of the cell, 2000 Dec;11(12):4309-21	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	Exponential phase cells cultivated in YPD	N/A
		PubMed Huang D et al., Molecular and cellular biology, 2002 Jul;22(14):5076-88	Indirect	Microarray analysis - WT vs TF Deletion	Positive	BY263	Yeast strains were grown in rich medium at 30°C to an optical density at 600 nm (OD600) of 0.5.	N/A
		PubMed Zhu H et al., Rapid communications in mass spectrometry : RCM, 2002;16(22):2115-23	Indirect	lacZ - wt vs TF mutant	Positive	S288c	Alkaline pH stress; Cells cultivated in SC medium at pH 8	N/A
		PubMed Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104	Direct	Chip-on-chip	N/A	S288c	N/A	N/A
		PubMed Chua G et al., Proceedings of the National Academy of Sciences of the United States of America, 2006 Aug 8;103(32):12045-50	Indirect	Microarray analysis - wt vs TF mutant	Positive	N/A	Cells grown in synthetic medium supplemented with 2% dextrose	N/A
			Indirect	Microarray analysis - wt vs TF overexpression	Positive	N/A	Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h	N/A
		PubMed Nishizawa M et al., PLoS biology, 2008 Dec 23;6(12):2817-30	Direct	Chip-on-chip	N/A	S288c	YNB medium without phosphate + 0,2microM (low Pi; phosphate limitation)	N/A
		PubMed Aow JS et al., Nucleic acids research, 2013 May;41(9):4877-87	Direct	ChIP	N/A	S288c	low phosphate	N/A
		PubMed Pimentel C et al., Biochimica et biophysica acta, 2014 Jun;1840(6):1977-86	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	2 mM CoSO4	N/A
		PubMed Serra-Cardona A et al., Molecular and cellular biology, 2014 Dec;34(24):4420-35	Indirect	Western blot - wt vs TF mutant	Positive	S288c	wt strain DBY746 and its pho4 derivative (RSC4) were transformed with centromeric plasmid pMM15-PHO84, which expresses the C-terminally HA-tagged version of Pho84. Cells were subjected to low-phosphate (100microM) stress and collected after the indicated periods, and the amount of Pho84 was assessed by using anti-HA antibodies	N/A
		PubMed He BZ et al., eLife, 2017 May 9;6():He BZ et al., eLife, 2017 May 9;6():	Indirect	RNA-seq analysis - WT vs TF Deletion	Positive	K699	100 ml of cell culture grown to OD600nm (600 nm) of 1.5.	11.8195096606715
			Indirect	RNA-seq analysis - WT vs TF Deletion	Positive	K699	Grown in phosphate-free SC medium (Phosphate starvation) at 30°C with shaking. All transcriptional profiling experiments were done in the pho80∆ background. Transcription factor gene deletion.	11.8195096606715
		PubMed Gurvich Y et al., PLoS biology, 2017 Dec;15(12):e2002039	Indirect	RNA-seq - wt vs TF mutant	Positive	BY4741 PHO4SA12346 allele	BY4741 PHO4SA12346 allele ; SC medium; Low P(i) concentration; 30.C; mid-log phase	N/A
		PubMed van Heusden GPH, Genomics, 2023 Sep;115(5):110672	Indirect	RNA-seq analysis - WT vs TF Deletion	Positive	BY4741	Grown overnight at 30 °C in phosphate-free YNB medium supplemented with 7.2 mM potassium phosphate (pH 5.8), 20 mg/L histidine, leucine, methionine, and uracil, and 50 mM KCl, then used to inoculate 50 mL cultures at OD620 = 0.1 and grown at 30 °C, 180 rpm to OD620 = 0.5. Transcription factor gene deletion.	2.02329765206832
		PubMed Huang Z et al., Metallomics : integrated biometal science, 2023 Dec 9;15(12):	Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	YPD medium	N/A
			Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	YPD medium + 100microgram/ml Al3+	N/A