Transcription Factor |
Target ORF/Genes |
References |
Evidence Code |
Evidence Experiment |
Association Type |
Strain |
Environmental Group |
Environmental Condition |
Log2FC |
|
Aft2p
|
YMR058W
|
PubMed
Blaiseau PL et al., The Journal of biological chemistry, 2001 Sep 7;276(36):34221-6Blaiseau PL et al., The Journal of biological chemistry, 2001 Sep 7;276(36):34221-6 |
Indirect |
Northern blot - wt vs TF mutant |
Positive |
CM3260 |
Ion/metal/phosphate/sulfur/vitamin availability |
Iron depletion; cells in log phase grown in iron and copper limiting medium without iron addition |
N/A |
| Indirect |
Northern blot - wt vs TF mutant |
Positive |
CM3260 |
Ion/metal/phosphate/sulfur/vitamin availability |
Iron repletion; cells in log phase grown in iron and copper limiting medium supplemented with 100 microM iron |
N/A |
| Indirect |
lacZ - wt vs TF mutant |
Positive |
CM3260 |
Ion/metal/phosphate/sulfur/vitamin availability |
Iron depletion; cells in log phase grown in iron and copper limiting medium without iron addition |
N/A |
| Indirect |
lacZ - wt vs TF mutant |
Positive |
CM3260 |
Ion/metal/phosphate/sulfur/vitamin availability |
Iron repletion; cells in log phase grown in iron and copper limiting medium supplemented with 100 microM iron |
N/A |
PubMed
Rutherford JC et al., Proceedings of the National Academy of Sciences of the United States of America, 2001 Dec 4;98(25):14322-7Rutherford JC et al., Proceedings of the National Academy of Sciences of the United States of America, 2001 Dec 4;98(25):14322-7 |
Direct |
EMSA |
N/A |
S288c |
In vitro |
in vitro |
N/A |
PubMed
Courel M et al., Molecular and cellular biology, 2005 Aug;25(15):6760-71Courel M et al., Molecular and cellular biology, 2005 Aug;25(15):6760-71 |
Indirect |
Northern blot WT vs TF mutant |
Negative |
Y14438 |
Ion/metal/phosphate/sulfur/vitamin availability |
Iron-depleted conditions - Defined medium (iron-limiting and copper-limiting yeast nitrogen base ); 30.C. |
N/A |
| Indirect |
Northern blot WT vs TF mutant |
Positive |
Y14438 |
Ion/metal/phosphate/sulfur/vitamin availability |
Iron- replete conditions - Defined medium (iron-limiting and copper-limiting yeast nitrogen base ) with 100uM ferric ammonium sulfate; 30.C. |
N/A |
| Indirect |
Northern blot - wt vs TF mutant |
Positive |
SCMC01 |
Ion/metal/phosphate/sulfur/vitamin availability |
Iron-depleted conditions - Defined medium (iron-limiting and copper-limiting yeast nitrogen base ); 30.C. |
N/A |
| Indirect |
Northern blot - wt vs TF mutant |
Positive |
SCMC01 |
Ion/metal/phosphate/sulfur/vitamin availability |
Iron- replete conditions - Defined medium (iron-limiting and copper-limiting yeast nitrogen base ) with 100uM ferric ammonium sulfate; 30.C. |
N/A |
| Indirect |
Microarray analysis - wt vs TF mutant |
Positive |
SCMC01 |
Ion/metal/phosphate/sulfur/vitamin availability |
Iron-depleted conditions - Defined medium (iron-limiting and copper-limiting yeast nitrogen base ); 30.C. |
N/A |
PubMed
Dubacq C et al., Molecular genetics and genomics : MGG, 2006 Feb;275(2):114-24Dubacq C et al., Molecular genetics and genomics : MGG, 2006 Feb;275(2):114-24 |
Indirect |
Microarray analysis - wt vs TF mutant |
Positive |
S288c |
Unstressed log-phase growth (control) |
YPD |
N/A |
PubMed
Gonçalves IR et al., Genetics, 2014 Jan;196(1):149-60Gonçalves IR et al., Genetics, 2014 Jan;196(1):149-60 |
Direct |
EMSA |
N/A |
S288c |
In vitro |
in vitro |
N/A |
PubMed
Pérez-Sampietro M et al., Scientific reports, 2016 Sep 13;6():32836Pérez-Sampietro M et al., Scientific reports, 2016 Sep 13;6():32836 |
Indirect |
Northern blot - wt vs TF mutant |
Positive |
S288c |
Stress |
5h treatment with Sodium selenite |
N/A |
| Indirect |
RNA-seq analysis - WT vs TF Deletion |
Positive |
W303-1A |
Carbon source quality/availability |
Grown at 30°C in YP (1% yeast extract, 2% bacto peptone, Difco, Detroit, MI) 2% raffinose medium. |
0.762043110403008 |
| Indirect |
RNA-seq analysis - WT vs TF Deletion |
Positive |
W303-1A |
Stress |
Grown in YPD medium until 1 × 10^7 cells/ml. Addition of sodium selenite (1mM) and sampled after 5h. Transcription factor gene deletion. |
0.762043110403008 |
PubMed
Pujol-Carrion N et al., Biochimica et biophysica acta. Molecular cell research, 2021 Apr;1868(5):118974Pujol-Carrion N et al., Biochimica et biophysica acta. Molecular cell research, 2021 Apr;1868(5):118974 |
Indirect |
lacZ - wt vs TF mutant |
Positive |
CML128 |
Unstressed log-phase growth (control) |
SD medium |
N/A |
| Indirect |
lacZ - wt vs TF mutant |
Positive |
CML128 |
Ion/metal/phosphate/sulfur/vitamin availability |
SD medium Fe depleted |
N/A |
| Indirect |
lacZ - wt vs TF mutant |
Positive |
CML128 |
Ion/metal/phosphate/sulfur/vitamin availability |
SD medium Fe repleted (10mM, 6h) |
N/A |