PubMed
Shu WJ et al., Nucleic acids research, 2020 Sep 4;48(15):8360-8373Shu WJ et al., Nucleic acids research, 2020 Sep 4;48(15):8360-8373
Indirect
RNA-seq - wt vs TF overexpression
Negative
BY4741
Stress
YPD medium; exponential phase treated with 100 nM Rapamycin for 2 h
N/A
PubMed
Couvillion M et al., eLife, 2022 May 16;11():Couvillion M et al., eLife, 2022 May 16;11():
Indirect
RNA-seq analysis - WT vs TF Deletion
Negative
BY4741
Unstressed log-phase growth (control)
Overnight cultures grown in YPD medium were diluted to OD600 = 0.05 in 1 L YPD and incubated at 30 °C with 200 rpm shaking to mid-log phase (OD600 = 0.6–0.8). Transcription factor gene deletion.
-0.85692910738501
PubMed
Glazier VE et al., mBio, 2023 Oct 31;14(5):e0152123Glazier VE et al., mBio, 2023 Oct 31;14(5):e0152123
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
BY4741
Unstressed log-phase growth (control)
Cultured in minimal medium (1.7 g/L YNB, 5 g/L ammonium sulfate, 20 g/L glucose, 80 mg/L histidine, 80 mg/L methionine, 80 mg/L uracil and 240 mg/L leucine) with 200 rpm orbital shaking at 30°C, pH 5.5, until the mid-exponential phase (OD600 = 0.6). Transcription factor gene deletion.
