PubMed
Wei M et al., PLoS genetics, 2009 May;5(5):e1000467Wei M et al., PLoS genetics, 2009 May;5(5):e1000467
Indirect
RT-PCR - wt vs TF mutant
Negative
S288c
SDC medium; mid-log phase
N/A
PubMed
Zhang N et al., The Journal of biological chemistry, 2010 Feb 26;285(9):6465-76Zhang N et al., The Journal of biological chemistry, 2010 Feb 26;285(9):6465-76
Indirect
Microarray analysis - TF Over-expression vs WT
Negative
CML376
Cells were grown to mid-exponential phase under doxycycline repression (A₆₀₀ ≈ 0.4), then washed and resuspended in medium with or without doxycycline for 6 h. Transcription factor tetO-GIS1 overexpression.
-0.59137869
PubMed
Glazier VE et al., mBio, 2023 Oct 31;14(5):e0152123Glazier VE et al., mBio, 2023 Oct 31;14(5):e0152123
Indirect
RNA-seq analysis - WT vs TF Deletion
Negative
BY4741
Cultured in minimal medium (1.7 g/L YNB, 5 g/L ammonium sulfate, 20 g/L glucose, 80 mg/L histidine, 80 mg/L methionine, 80 mg/L uracil and 240 mg/L leucine) with 200 rpm orbital shaking at 30°C, pH 5.5, until the mid-exponential phase (OD600 = 0.6). Transcription factor gene deletion.
