PubMed
Reimand J et al., Nucleic acids research, 2010 Aug;38(14):4768-77Reimand J et al., Nucleic acids research, 2010 Aug;38(14):4768-77
Indirect
Microarray analysis - WT vs TF Deletion
Positive
BY4741; S288C; R1158
Standard YPD conditions Dataset ewxtracted from The Longhorn Array Database (LAD). Transcription factor gene deletion.
0.78867504663105
PubMed
Ensinck I et al., eLife, 2023 Jul 25;12():Ensinck I et al., eLife, 2023 Jul 25;12():
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
FW1511
4 h: RNA-seq performed under sporulation conditions in SK1 background. Overnight YPD cultures diluted to OD600 0.5 in pre-sporulation medium (SPS: 0.5% yeast extract, 1% peptone, 0.17% YNB, 1% potassium acetate, 0.5% ammonium sulfate, 1% glucose) for 16-18 h at 30°C. Washed and transferred to 1% potassium acetate (SPO medium) at OD600 1.9. Samples collected at 4 h post-transfer to SPO.
2.237416
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
FW1511
6 h: RNA-seq performed under sporulation conditions in SK1 background. Overnight YPD cultures diluted to OD600 0.5 in pre-sporulation medium (SPS: 0.5% yeast extract, 1% peptone, 0.17% YNB, 1% potassium acetate, 0.5% ammonium sulfate, 1% glucose) for 16-18 h at 30°C. Washed and transferred to 1% potassium acetate (SPO medium) at OD600 1.9. Samples collected at 6 h post-transfer to SPO.
3.670601
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
FW1511
8 h: RNA-seq performed under sporulation conditions in SK1 background. Overnight YPD cultures diluted to OD600 0.5 in pre-sporulation medium (SPS: 0.5% yeast extract, 1% peptone, 0.17% YNB, 1% potassium acetate, 0.5% ammonium sulfate, 1% glucose) for 16-18 h at 30°C. Washed and transferred to 1% potassium acetate (SPO medium) at OD600 1.9. Samples collected at 8 h post-transfer to SPO.
