PubMed
Shu WJ et al., Nucleic acids research, 2020 Sep 4;48(15):8360-8373Shu WJ et al., Nucleic acids research, 2020 Sep 4;48(15):8360-8373
Indirect
RNA-seq analysis - WT vs TF Deletion
Negative
BY4741
Steady state carbon-limited chemostat cultures operated at a dilution rate of 0.05 per h by continuous addition of medium (feed concentration of glucose, 10 g/liter) at 30°C. pH controlled at 5, aerated with 0.5 volume of air/volume of liquid/min and with 800 rpm agitation. Transcription factor gene deletion.
-1.01158669213112
Indirect
RNA-seq analysis - WT vs TF Deletion
Negative
BY4741
Ethanol medium - Overexpression media lacked leucine to ensure plasmid selection. The cultures grown for 70 h in ethanol medium were diluted twice with a fresh medium to ensure continuous growth.
-0.503581225901785
Indirect
RNA-seq analysis - WT vs TF Deletion
Negative
BY4741
Grown in YPD to OD600 of 0.8 at 30 ºC. Transcription factor gene deletion.
-1.01158669213112
Indirect
RNA-seq analysis - WT vs TF Deletion
Negative
BY4741
Grown in YPD to OD600 of 0.8 at 30 ºC. Treated with rapamycin for 2h (100 nM). Transcription factor gene deletion.
-0.503581225901785
PubMed
Fang W et al., Cell reports, 2022 Apr 12;39(2):110672Fang W et al., Cell reports, 2022 Apr 12;39(2):110672
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
CEN.PK
Yeast cells grown to log phase in YPL medium from 0.1 OD with switch to SL minimal medium for 1h. Transcription factor gene deletion.
1.12945856334036
PubMed
Glazier VE et al., mBio, 2023 Oct 31;14(5):e0152123Glazier VE et al., mBio, 2023 Oct 31;14(5):e0152123
Indirect
RNA-seq analysis - WT vs TF Deletion
Negative
BY4741
Cultured in minimal medium (1.7 g/L YNB, 5 g/L ammonium sulfate, 20 g/L glucose, 80 mg/L histidine, 80 mg/L methionine, 80 mg/L uracil and 240 mg/L leucine) with 200 rpm orbital shaking at 30°C, pH 5.5, until the mid-exponential phase (OD600 = 0.6). Transcription factor gene deletion.
