PubMed
Garland SA et al., Journal of molecular biology, 1999 Dec 10;294(4):897-907Garland SA et al., Journal of molecular biology, 1999 Dec 10;294(4):897-907
Indirect
Northern blot - wt vs TF overexpression
Positive
S288c
constitutively active AFT1 allele; cells grown in YPD to mid-log phase (basal conditions)
N/A
PubMed
Dubacq C et al., Molecular genetics and genomics : MGG, 2006 Feb;275(2):114-24Dubacq C et al., Molecular genetics and genomics : MGG, 2006 Feb;275(2):114-24
Indirect
Microarray analysis - wt vs TF mutant
Positive
S288c
YPD medium + 200mM hydroxyurea
N/A
PubMed
Reimand J et al., Nucleic acids research, 2010 Aug;38(14):4768-77Reimand J et al., Nucleic acids research, 2010 Aug;38(14):4768-77
Indirect
Microarray analysis - WT vs TF Deletion
Negative
BY4741; S288C; R1158
Standard YPD conditions Dataset ewxtracted from The Longhorn Array Database (LAD). Transcription factor gene deletion.