PubMed
Reimand J et al., Nucleic acids research, 2010 Aug;38(14):4768-77Reimand J et al., Nucleic acids research, 2010 Aug;38(14):4768-77
Indirect
Microarray analysis - WT vs TF Deletion
Positive
BY4741; S288C; R1158
Standard YPD conditions Dataset ewxtracted from The Longhorn Array Database (LAD). Transcription factor gene deletion.
0.35709788908704
PubMed
Shu WJ et al., Nucleic acids research, 2020 Sep 4;48(15):8360-8373Shu WJ et al., Nucleic acids research, 2020 Sep 4;48(15):8360-8373
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
BY4741
Ethanol medium - Overexpression media lacked leucine to ensure plasmid selection. The cultures grown for 70 h in ethanol medium were diluted twice with a fresh medium to ensure continuous growth.
0.399144526458341
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
BY4741
Grown in YPD to OD600 of 0.8 at 30 ÂșC. Treated with rapamycin for 2h (100 nM). Transcription factor gene deletion.