PubMed
Chua G et al., Proceedings of the National Academy of Sciences of the United States of America, 2006 Aug 8;103(32):12045-50Chua G et al., Proceedings of the National Academy of Sciences of the United States of America, 2006 Aug 8;103(32):12045-50
Indirect
Microarray analysis - wt vs TF overexpression
Positive
N/A
Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h
N/A
PubMed
Zhang N et al., The Journal of biological chemistry, 2010 Feb 26;285(9):6465-76Zhang N et al., The Journal of biological chemistry, 2010 Feb 26;285(9):6465-76
Indirect
Microarray analysis - TF Over-expression vs WT
Positive
CML376
Cells were grown to mid-exponential phase under doxycycline repression (A₆₀₀ ≈ 0.4), then washed and resuspended in medium with or without doxycycline for 6 h. Transcription factor tetO-GIS1 overexpression.
0.3759554
PubMed
Glazier VE et al., mBio, 2023 Oct 31;14(5):e0152123Glazier VE et al., mBio, 2023 Oct 31;14(5):e0152123
Indirect
RNA-seq analysis - WT vs TF Deletion
Negative
BY4741
Cultured in minimal medium (1.7 g/L YNB, 5 g/L ammonium sulfate, 20 g/L glucose, 80 mg/L histidine, 80 mg/L methionine, 80 mg/L uracil and 240 mg/L leucine) with 200 rpm orbital shaking at 30°C, pH 5.5, until the mid-exponential phase (OD600 = 0.6). Transcription factor gene deletion.
