PubMed
Chua G et al., Proceedings of the National Academy of Sciences of the United States of America, 2006 Aug 8;103(32):12045-50Chua G et al., Proceedings of the National Academy of Sciences of the United States of America, 2006 Aug 8;103(32):12045-50
Indirect
Microarray analysis - wt vs TF overexpression
Negative
N/A
Carbon source quality/availability
Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h
N/A
PubMed
Kelley R et al., PLoS genetics, 2009 May;5(5):e1000488Kelley R et al., PLoS genetics, 2009 May;5(5):e1000488
Indirect
Microarray analysis - WT vs TF Deletion
Positive
BY4741
Stress
Single colony overnight growth in 10 mL of YPD at 30°C resuspended in 100 mL of YPD media at an OD600 of 0.1 and placed in an orbital shaker at 30°C. Yeast cells grown to OD600 0.6 and treated with 0.1 mM H2O2 for 45 minutes. Transcription factor gene deletion.
1.336193
PubMed
van der Horst SC et al., Nature communications, 2025 Feb 6;16(1):1416van der Horst SC et al., Nature communications, 2025 Feb 6;16(1):1416
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
W303
Cell cycle/morphology
60 min: RNA-seq performed in YPAD medium. Overnight cultures were diluted in fresh YPAD and grown for 3 h. Cells were then synchronized in G1 by α-factor treatment for 2 h, washed once in YPAD, and released into YPAD containing 200 mM hydroxyurea (HU). Samples were collected at t=60min: 60 min after release into HU (replication stress condition)
