S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition	Log2FC
Ume6p	YPL160W	PubMed Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104	Direct	Chip-on-chip	N/A	S288c	YPD medium; mid-log phase	N/A
		PubMed Reimand J et al., Nucleic acids research, 2010 Aug;38(14):4768-77	Indirect	Microarray analysis - WT vs TF Deletion	Positive	BY4741; S288C; R1158	Standard YPD conditions Dataset ewxtracted from The Longhorn Array Database (LAD). Transcription factor gene deletion.	0.35060236175702
		PubMed Lardenois A et al., Molecular genetics and genomics : MGG, 2015 Oct;290(5):2031-46	Indirect	Microarray analysis - WT vs TF Deletion	Positive	SK1	Grown in sporulation medium (SPII for 10 h) under standard conditions. Transcription factor gene deletion.	0.944153
			Indirect	Microarray analysis - WT vs TF Deletion	Positive	SK1	Grown in sporulation medium (SPII for 8 h) under standard conditions. Transcription factor gene deletion.	0.990245
			Indirect	Microarray analysis - WT vs TF Deletion	Positive	SK1	Grown in rich media with acetate (YPA) under standard conditions. Transcription factor gene deletion.	1.585476
		PubMed Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314	Direct	ChIP-exo	N/A	BY4741	YPD medium, 25.C	N/A
		PubMed Harris A et al., Genetics, 2023 Oct 4;225(2):	Indirect	RNA-seq analysis - WT vs TF Deletion	Positive	SK1	Mitotic, 30min: Cells were in mitotic, log-phase growth. They were grown in YPD (1% yeast extract, 2% peptone, 2% dextrose, 22.4 mg/L uracil, 80 mg/L tryptophan) for ~24 h to saturation, then back-diluted into fresh YPD to OD600 = 0.2 and grown ~3 h to log phase (OD600 ≥ 0.5). Once in log phase, β-estradiol (40 nM) was added, followed 30 min later by auxin (200 µM) at t=0min. Sampled at 30 min.	0.2126
			Indirect	RNA-seq analysis - WT vs TF Under-expression	Positive	SK1	Mitotic, 15min: Cells were in mitotic, log-phase growth. They were grown in YPD (1% yeast extract, 2% peptone, 2% dextrose, 22.4 mg/L uracil, 80 mg/L tryptophan) for ~24 h to saturation, then back-diluted into fresh YPD to OD600 = 0.2 and grown ~3 h to log phase (OD600 ≥ 0.5). Once in log phase, β-estradiol (40 nM) was added, followed 30 min later by auxin (200 µM) at t=0min. Sampled at 15 min.	0.148754
			Indirect	RNA-seq analysis - WT vs TF Under-expression	Positive	SK1	Mitotic, 60min: Cells were in mitotic, log-phase growth. They were grown in YPD (1% yeast extract, 2% peptone, 2% dextrose, 22.4 mg/L uracil, 80 mg/L tryptophan) for ~24 h to saturation, then back-diluted into fresh YPD to OD600 = 0.2 and grown ~3 h to log phase (OD600 ≥ 0.5). Once in log phase, β-estradiol (40 nM) was added, followed 30 min later by auxin (200 µM) at t=0min. Sampled at 60 min.	0.22789