PubMed
Vizoso-Vázquez A et al., Applied microbiology and biotechnology, 2012 Apr;94(1):173-84Vizoso-Vázquez A et al., Applied microbiology and biotechnology, 2012 Apr;94(1):173-84
Indirect
Microarray analysis - WT vs TF Deletion
Positive
BY4741
Grown in SC medium with defined amino acid supplements and 2% glucose at 30 °C, in Erlenmeyer flasks filled to 40% volume and shaken at 250 rpm. Cells shifted from aerobic to hypoxic conditions at OD600 0.8 by nitrogen bubbling for 3 hours. Transcription factor gene deletion in hypoxic condition.
N/A
PubMed
Krakowiak J et al., eLife, 2018 Feb 2;7():Krakowiak J et al., eLife, 2018 Feb 2;7():
Indirect
Microarray analysis - WT vs TF Deletion
Positive
W303a
Grown overnight in complete SD medium with specific amino acid supplements and 2% glucose, then diluted to OD600 0.4 in fresh medium and cultured at 30 °C with shaking until mid-log phase (OD600 0.6). Transcription factor gene deletion.
0.82737
Indirect
Microarray analysis - WT vs TF Deletion
Positive
W303a
Grown overnight in complete SD medium with specific amino acid supplements and 2% glucose, then diluted to OD600 0.4 in fresh medium and cultured at 30 °C with shaking until mid-log phase (OD600 0.6). Cisplatin treatment (600 μM in DMSO), incubated for 4 hours at 30 °C in the dark with agitation. Transcription factor gene deletion.
