PubMed
Lee TI et al., Science (New York, N.Y.), 2002 Oct 25;298(5594):799-804Lee TI et al., Science (New York, N.Y.), 2002 Oct 25;298(5594):799-804
Direct
Chip-on-chip
N/A
S288c
YPD medium; mid-log phase
N/A
PubMed
Sarode N et al., Eukaryotic cell, 2011 Nov;10(11):1516-26Sarode N et al., Eukaryotic cell, 2011 Nov;10(11):1516-26
Direct
ChIP
N/A
S288c
Iron exposure
N/A
Indirect
Microarray analysis - WT vs TF Deletion
Positive
DY150-del-ccc1
Cells were grown overnight in CMM and then incubated with or without 500 μm ferrous iron for another 2 h.
N/A
PubMed
Pimentel C et al., PloS one, 2012;7(5):e37434Pimentel C et al., PloS one, 2012;7(5):e37434
Indirect
Microarray analysis - WT vs TF Deletion
Positive
BY4742
Grown in synthetic media (SC: 0.67% ammonium sulphate-yeast nitrogen base without amino acids, 2% glucose, supplemented with the appropriate selective amino acids). Early log-phase cultures (A600=0.4) were exposed to 2mM of FeSO4 for 20 min. Transcription factor gene deletion.
2.172447
Indirect
Microarray analysis - WT vs TF Deletion
Positive
BY4742
Grown in synthetic media (SC: 0.67% ammonium sulphate-yeast nitrogen base without amino acids, 2% glucose, supplemented with the appropriate selective amino acids). Early log-phase cultures (A600=0.4) were exposed to 2mM of FeSO4 for 60 min. Transcription factor gene deletion.
1.553522
PubMed
Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314