PubMed
Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314
Direct
ChIP-exo
N/A
BY4741
Unstressed log-phase growth (control)
YPD medium, 25.C
N/A
PubMed
Horkai D et al., PLoS biology, 2023 Aug;21(8):e3002245Horkai D et al., PLoS biology, 2023 Aug;21(8):e3002245
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
MEP
Carbon source quality/availability
YPD,48h: Cells were grown in YP + 2% glucose (YPD) at 30°C, 200 rpm and mother cells were harvested 48h after estradiol addition (added at 2h post-inoculation).
0.794331
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
MEP
Unstressed log-phase growth (control)
YPD,log: Cells were grown in YP + 2% glucose (YPD) at 30°C, 200 rpm and harvested at log phase as a young control.
1.60251
PubMed
van der Horst SC et al., Nature communications, 2025 Feb 6;16(1):1416van der Horst SC et al., Nature communications, 2025 Feb 6;16(1):1416
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
W303
Cell cycle/morphology
0 min: RNA-seq performed in YPAD medium. Overnight cultures were diluted in fresh YPAD and grown for 3 h. Cells were then synchronized in G1 by α-factor treatment for 2 h, washed once in YPAD, and released into YPAD containing 200 mM hydroxyurea (HU). Samples were collected at t=0min: immediately after α-factor synchronization (pre-HU release)