PubMed
Shu WJ et al., Nucleic acids research, 2020 Sep 4;48(15):8360-8373Shu WJ et al., Nucleic acids research, 2020 Sep 4;48(15):8360-8373
Indirect
RNA-seq analysis - WT vs TF Deletion
Negative
BY4741
Steady state carbon-limited chemostat cultures operated at a dilution rate of 0.05 per h by continuous addition of medium (feed concentration of glucose, 10 g/liter) at 30°C. pH controlled at 5, aerated with 0.5 volume of air/volume of liquid/min and with 800 rpm agitation. Transcription factor gene deletion.
-0.622870802585994
Indirect
RNA-seq analysis - WT vs TF Deletion
Negative
BY4741
Ethanol medium - Overexpression media lacked leucine to ensure plasmid selection. The cultures grown for 70 h in ethanol medium were diluted twice with a fresh medium to ensure continuous growth.
-0.313086566804909
Indirect
RNA-seq analysis - WT vs TF Deletion
Negative
BY4741
Grown in YPD to OD600 of 0.8 at 30 ºC. Transcription factor gene deletion.
-0.622870802585994
Indirect
RNA-seq analysis - WT vs TF Deletion
Negative
BY4741
Grown in YPD to OD600 of 0.8 at 30 ºC. Treated with rapamycin for 2h (100 nM). Transcription factor gene deletion.
-0.313086566804909
PubMed
Fang W et al., Cell reports, 2022 Apr 12;39(2):110672Fang W et al., Cell reports, 2022 Apr 12;39(2):110672
Indirect
RNA-seq analysis - WT vs TF Deletion
Negative
CEN.PK
Yeast cells grown to log phase in YPL medium from 0.1 OD. Transcription factor gene deletion.
-0.322420028590166
PubMed
Glazier VE et al., mBio, 2023 Oct 31;14(5):e0152123Glazier VE et al., mBio, 2023 Oct 31;14(5):e0152123
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
BY4741
Cultured in minimal medium (1.7 g/L YNB, 5 g/L ammonium sulfate, 20 g/L glucose, 80 mg/L histidine, 80 mg/L methionine, 80 mg/L uracil and 240 mg/L leucine) with 200 rpm orbital shaking at 30°C, pH 5.5, until the mid-exponential phase (OD600 = 0.6). Transcription factor gene deletion.
