PubMed
Shu WJ et al., Nucleic acids research, 2020 Sep 4;48(15):8360-8373Shu WJ et al., Nucleic acids research, 2020 Sep 4;48(15):8360-8373
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
BY4741
Ethanol medium - Overexpression media lacked leucine to ensure plasmid selection. The cultures grown for 70 h in ethanol medium were diluted twice with a fresh medium to ensure continuous growth.
0.656484520288
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
BY4741
Grown in YPD to OD600 of 0.8 at 30 ÂșC. Treated with rapamycin for 2h (100 nM). Transcription factor gene deletion.
0.656484520288
PubMed
Fang W et al., Cell reports, 2022 Apr 12;39(2):110672Fang W et al., Cell reports, 2022 Apr 12;39(2):110672
Indirect
RNA-seq analysis - WT vs TF Deletion
Negative
CEN.PK
Yeast cells grown to log phase in YPL medium from 0.1 OD with switch to SL minimal medium for 1h. Transcription factor gene deletion.